期刊
CELL RESEARCH
卷 22, 期 10, 页码 1453-1466出版社
INST BIOCHEMISTRY & CELL BIOLOGY
DOI: 10.1038/cr.2012.88
关键词
embryo; HCO3-; miR-125b; CFTR; sAC
类别
资金
- National Basic Research Program of China (973 program) [2012CB944900]
- National Natural Science Foundation of China [30973209]
- Focused Investment Scheme and Li Ka Shing Institute of Health Sciences of The Chinese University of Hong Kong, Fundamental Research Funds for the Central Universities (Jinan University)
- Morning-side Foundation
Although HCO3- is known to be required for early embryo development, its exact role remains elusive. Here we report that HCO3- acts as an environmental cue in regulating miR-125b expression through CFTR-mediated influx during preimplantation embryo development. The results show that the effect of HCO3- on preimplantation embryo development can be suppressed by interfering the function of a HCO3--conducting channel, CFTR, by a specific inhibitor or gene knockout. Removal of extracellular HCO3- or inhibition of CFTR reduces miR-125b expression in 2 cell-stage mouse embryos. Knockdown of miR-125b mimics the effect of HCO3- removal and CFTR inhibition, while injection of miR-125b precursor reverses it. Downregulation of miR-125b upregulates p53 cascade in both human and mouse embryos. The activation of miR-125b is shown to be mediated by sAC/PKA-dependent nuclear shuttling of NF-kappa B. These results have revealed a critical role of CFTR in signal transduction linking the environmental HCO3- to activation of miR-125b during preimplantation embryo development and indicated the importance of ion channels in regulation of miRNAs.
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