期刊
JOURNAL OF APPLIED PHYCOLOGY
卷 15, 期 6, 页码 451-456出版社
SPRINGER
DOI: 10.1023/B:JAPH.0000004298.89183.e5
关键词
alga; Dunaliella salina; halotolerance; hepatitis B surface antigen gene; stable expression
A stable transformation system for the expression of foreign genes in the unicellular marine green alga Dunaliella salina was established. Among five antibiotics, 60 mug mL(-1) chloramphenicol completely inhibited growth. Of five promoters tested, the ubiquitin-Omega promoter yielded the highest beta-glucuronidase (GUS) activity. The hepatitis B surface antigen ( HBsAg) gene was introduced into the cells by using electroporation. PCR and Southern blot analysis amd it was shown that the gene was integrated into the genome. The stable expression of HBsAg protein was confirmed by enzyme-linked immunosorbent assay ( ELISA) and Western blot analysis. The introduced DNA and HBsAg expression were maintained stable for at least 60 generations in medium devoid of chloramphenicol. This is an important step toward the production of useful foreign proteins in the alga.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据