期刊
CELL RESEARCH
卷 22, 期 1, 页码 208-218出版社
INST BIOCHEMISTRY & CELL BIOLOGY
DOI: 10.1038/cr.2011.175
关键词
direct conversion; neural stem cell; multipotent; transdifferentiation; transplantation
类别
资金
- National Science Foundation of China [90919060, 31070946]
- Chinese Academy of Sciences [XDA01020100]
- China National Basic Research Program [2007CB947702, 2011CBA01101, 2011CB965103]
Multipotent neural stem/progenitor cells hold great promise for cell therapy. The reprogramming of fibroblasts to induced pluripotent stem cells as well as mature neurons suggests a possibility to convert a terminally differentiated somatic cell into a multipotent state without first establishing pluripotency. Here, we demonstrate that sertoli cells derived from mesoderm can be directly converted into a multipotent state that possesses neural stem/progenitor cell properties. The induced neural stem/progenitor cells (iNSCs) express multiple NSC-specific markers, exhibit a global gene-expression profile similar to normal NSCs, and are capable of self-renewal and differentiating into glia and electrophysiologically functional neurons. iNSC-derived neurons stain positive for tyrosine hydroxylase (TH), gamma-aminobutyric acid, and choline acetyltransferase. In addition, iNSCs can survive and generate synapses following transplantation into the dentate gyrus. Generation of iNSCs may have important implications for disease modeling and regenerative medicine.
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