期刊
CELL RESEARCH
卷 21, 期 10, 页码 1436-1451出版社
INST BIOCHEMISTRY & CELL BIOLOGY
DOI: 10.1038/cr.2011.136
关键词
Cubitus interruptus; Drosophila; Fused; Hedgehog; morphogen
类别
资金
- National Natural Science Foundation of China [30623003, 30971647, 30971646]
- National Key Scientific Program of China [2011CB943902, 2010CB912100]
- Shanghai Pujiang Program [09PJ1410900]
- Chinese Academy of Sciences [XDA01010405, XDA01010406]
The Hedgehog (Hh) family of secreted proteins is essential for development in both vertebrates and invertebrates. As one of main morphogens during metazoan development, the graded Hh signal is transduced across the plasma membrane by Smoothened (Smo) through the differential phosphorylation of its cytoplasmic tail, leading to pathway activation and the differential expression of target genes. However, how Smo transduces the graded Hh signal via the Costal2 (Cos2)/Fused(Fu) complex remains poorly understood. Here we present a model of the cell response to a Hh gradient by translating Smo phosphorylation information to Fu dimerization and Cubitus interruptus (Ci) nuclear localization information. Our findings suggest that the phosphorylated C-terminus of Smo recruits the Cos2/Fu complex to the membrane through the interaction between Smo and Cos2, which further induces Fu dimerization. Dimerized Fu is phosphorylated and transduces the Hh signal by phosphorylating Cos2 and Suppressor of Fu (Su(fu)). We further show that this process promotes the dissociation of the full-length Ci (Ci155) and Cos2 or Su(fu), and results in the translocation of Ci155 into the nucleus, activating the expression of target genes.
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