期刊
JOURNAL OF PROTEOME RESEARCH
卷 2, 期 6, 页码 581-588出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr034030n
关键词
glycoprotein; glycosylation; beta-trace; alpha-1-antitrypsin; cerebrospinal fluid; CSF; 2D-PAGE; glycoproteomics; proteomics; infrared multiphoton dissociation; IRMPD; electrospray; ESI; MS/MS; tandem MS; FT-ICR; FTMS; Fourier transform; ion cyclotron resonance; ICR
The feasibility of global glycoprotein analysis by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and infrared multiphoton dissociation (IRMPD) tandem mass spectrometry is demonstrated. Combined 2D gel glycoprotein separation and visualization, in-gel digestion, and accurate (<10 ppm) mass measurement allowed identification of human glycoproteins and revealed differences in glycosylation. IRMPD obviates the need for glycan release, which prevents sample dispersal, and allows the assignment of glycan structures to specific sites of N-glycosylation.
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