期刊
PHYTOCHEMICAL ANALYSIS
卷 14, 期 6, 页码 347-351出版社
WILEY
DOI: 10.1002/pca.725
关键词
HPLC-MS; 1-aminocyclopropane-1-carboxylic acid; cyclopropane-1,1-dicarboxylic acid; 1-aminocyclopropane-1carboxylic acid oxidase inhibitor; ethylene; tissue culture; tomato; Lycopersicum esculentum
Varying concentrations of cyclopropane-1,1-dicarboxylic acid (CDA), an inhibitor of 1-aminocyclopropane-1-carboxylic acid oxidase, added to the solid culture medium of tomato nodal shoot segments resulted in a reduction in the level of endogenous ethylene according to the concentration of inhibitor applied. Following treatment with inhibitor, plants were homogenised and the concentrations of CDA and of 1-aminocyclopropane-1-carboxylic acid (ACC) were measured simultaneously in the resulting juice using an HPLC-ESI/MS-MS method. The levels of CDA and ACC measured in the plant tissues were associated with the concentration of inhibitor added to the solid medium. The HPLC-ESI/MS-MS method described produced limits of detection of 0.8 pmol for ACC and of 4 pmol for CDA. Copyright (C) 2003 John Wiley Sons, Ltd.
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