期刊
JOURNAL OF PROTEOME RESEARCH
卷 2, 期 6, 页码 589-597出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr034018v
关键词
ion mobility separation; tandem mass spectrometry; nanoLC/IMS/MSMS; protein identification
资金
- NIGMS NIH HHS [1R01GM-59145-03] Funding Source: Medline
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM059145] Funding Source: NIH RePORTER
A prototype linear octopole ion trap/ion mobility/tandem mass spectrometer has been coupled with a nanoflow liquid chromatography separation approach and used to separate and characterize a complicated peptide mixture from digestion of soluble proteins extracted from human urine. In this approach, two dimensions of separation (nanoflow liquid chromatography and ion mobility) are followed by collision induced dissociation (CID) and mass spectrometry (MS) analysis. From a preliminary analysis of the most intense CID-MS features in a part of the dataset, it is possible to assign 27 pepticle ions which correspond to 13 proteins. The data contain many additional CID-MS features for less intense ions. A limited discussion of these features and their potential utility in identifying complicated pepticle mixtures required for proteomics study is presented.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据