期刊
CELL RESEARCH
卷 21, 期 1, 页码 205-212出版社
INST BIOCHEMISTRY & CELL BIOLOGY
DOI: 10.1038/cr.2010.172
关键词
Oct4; iPSCs; reprogramming; stem cell; BMPs; MET
类别
资金
- National Natural Science Foundation of China [30630039, 30725012, 90813033]
- MOST [2009CB941102]
- Ministry of Science and Technology [2010DFB30430]
- Chinese Academy of Sciences [KSCX2-YW-R-221, KSCX2-YW-R-244]
- Chinese Academy of Sciences/SAFEA International Partnership Program for Creative Research Teams
Generation of induced pluripotent stem cells by defined factors has become a useful model to investigate the mechanism of reprogramming and cell fate determination. However, the precise mechanism of factor-based reprogramming remains unclear. Here, we show that Klf4 mainly acts at the initial phase of reprogramming to initiate mesenchymal-to-epithelial transition and can be functionally replaced by bone morphogenetic proteins (BMPs). BMPs boosted the efficiency of Oct4/Sox2-mediated reprogramming of mouse embryonic fibroblasts (MEFs) to similar to 1%. BMPs also promoted single-factor Oct4-based reprogramming of MEFs and tail tibial fibroblasts. Our studies clarify the contribution of Klf4 in reprogramming and establish Oct4 as a singular setter of pluripotency in differentiated cells.
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