期刊
CELL RESEARCH
卷 19, 期 10, 页码 1205-1216出版社
INST BIOCHEMISTRY & CELL BIOLOGY
DOI: 10.1038/cr.2009.84
关键词
EMB1303; albino; chloroplast development; embryogenesis; Arabidopsis
类别
资金
- National Natural Science Foundation of China [30670181, 30770202]
- Ministry of Science and Technology of China [2009CB119103]
- Program for New Century Excellent Talents in University [NCET-05-0124]
- Key Project of Chinese Ministry of Education [106037]
To understand the molecular mechanisms underlying chloroplast development, we isolated and characterized the albino mutant emb1303-1 in Arabidopsis. The mutant displayed a severe dwarf phenotype with small albino rosette leaves and short roots on a synthetic medium containing sucrose. It is pigment-deficient and seedling lethal when grown in soil. Embryo development was delayed in the mutant, although seed germination was not significantly impaired. The plastids of emb1303-1 were arrested in early developmental stages without the classical stack of thylakoid membrane. Genetic and molecular analyses uncovered that the EMB1303 gene encodes a novel chloroplast-localized protein. Microarray and RT-PCR analyses revealed that a number of nuclear- and plastid-encoded genes involved in photosynthesis and chloroplast biogenesis were substantially downregulated in the mutant. Moreover, the accumulation of several major chloroplast proteins was severely compromised in emb1303-1. These results suggest that EMB1303 is essential for chloroplast development.
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