期刊
CELL RESEARCH
卷 18, 期 10, 页码 997-1006出版社
INST BIOCHEMISTRY & CELL BIOLOGY
DOI: 10.1038/cr.2008.282
关键词
serum-microRNA; expression profile; fingerprint; blood-based biomarker; Solexa; cancers; diabetes
类别
资金
- National Natural Science Foundation of China [30225037, 30471991, 30570731]
- National Basic Research Program of China [2006CB503909, 2004CB518603]
- Natural Science Foundation of Jiangsu Province [BK2004082, BK2006714]
Dysregulated expression of microRNAs (miRNAs) in various tissues has been associated with a variety of diseases, including cancers. Here we demonstrate that miRNAs are present in the serum and plasma of humans and other animals such as mice, rats, bovine fetuses, calves, and horses. The levels of miRNAs in serum are stable, reproducible, and consistent among individuals of the same species. Employing Solexa, we sequenced all serum miRNAs of healthy Chinese subjects and found over 100 and 91 serum miRNAs in male and female subjects, respectively. We also identified specific expression patterns of serum miRNAs for lung cancer, colorectal cancer, and diabetes, providing evidence that serum miRNAs contain fingerprints for various diseases. Two non-small cell lung cancer-specific serum miRNAs obtained by Solexa were further validated in an independent trial of 75 healthy donors and 152 cancer patients, using quantitative reverse transcription polymerase chain reaction assays. Through these analyses, we conclude that serum miRNAs can serve as potential biomarkers for the detection of various cancers and other diseases.
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