期刊
JOURNAL OF PROTEOME RESEARCH
卷 2, 期 1, 页码 69-79出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr025562r
关键词
melanoma; proteomics; melanosome
资金
- NIGMS NIH HHS [GM 37537] Funding Source: Medline
- DIVISION OF BASIC SCIENCES - NCI [Z01BC009100] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM037537] Funding Source: NIH RePORTER
Melanin is a heterogeneous biopolymer produced only by specific cells termed melanocytes, which synthesize and deposit the pigment in specialized membrane-bound organelles known as melanosomes. Although melanosomes have been suspected of being closely related to lysosomes and platelets, the total number of melanosomal proteins is still unknown. Thus far, six melanosome-specific proteins have been identified, and the challenge is to characterize the complete proteome of the melanosome to further understand its mechanism of biogenesis. In this report, we used mass spectrometry and subcellular fractionation to identify protein components of early melanosomes. Using this approach, we have identified all 6 of the known melanosome-specific proteins, 56 proteins that are shared with other organelles, and confirmed the presence of 6 novel melanosomal proteins using western blotting and by immunohistochemistry.
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