期刊
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS
卷 41, 期 1, 页码 43-54出版社
WILEY
DOI: 10.1002/em.10129
关键词
polyphenon E; salmonella test; Tk; micronucleus; lacl/cll transgenic; mutation
资金
- NCI NIH HHS [N01 CN 75024] Funding Source: Medline
- DIVISION OF CANCER PREVENTION AND CONTROL [N01CN075024] Funding Source: NIH RePORTER
The potential health benefits of green tea continue to attract public and scientific interests and are attributed in part to polyphenolic catechin constituents. Polyphenon E (Poly E) is a decaffeinated green tea catechin mixture containing about 50% epigallocatechin gallate and 30% other catechins. We evaluated the toxicity and genotoxicity of Poly E by using two in vitro assays: bacterial mutagenesis in a Salmonella typhimurium-E. coli assay and the L5178Y mouse lymphoma cell thymidine kinase (Tk) gene mutation assay. In addition, we used two in vivo genotoxicity assays: the mouse micronucleus assay and the Big Blue cll transgenic mouse mutation assay. Repeat-dose toxicity evaluations were performed in mice in parallel with the Big Blue transgenic mutation assays. No significant increases in the revertant colonies were found in increase in the mutant frequency (MF) at the A locus was observed in the mouse lymphoma test system. We observed toxicity in mice when Poly E was administered at doses of 2,000 mg/kg/day. Lower doses produced no significant increases in micronucleated erythrocytes in the bone marrow of Swiss-Webster mice and no significant increases in cll transgene MF in the liver, lung, or spleen compared with controls. These results indicate that Poly E, although toxic at high doses (2,000 mg/kg/day), poses minimal genotoxic concern. In addition, these studies highlight the importance of using both in vitro and in vivo systems in genetic toxicity screening of pharmaceuticals before they are administered to humans. (C) 2003 Wiley-Liss, Inc.
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