期刊
CELL PROLIFERATION
卷 46, 期 1, 页码 23-37出版社
WILEY-BLACKWELL
DOI: 10.1111/cpr.12001
关键词
-
类别
资金
- Academy of Sciences of the Czech Republic [AV0Z50390703, AV0Z50390512]
- Grant Agency of the Academy of Sciences [IAA500390702]
- Grant Agency of the Charles University [96610, 97110, 330611, 384311, 164010, 626011]
- Grant Agency of the Czech Republic [P304/10/1307]
- Internal Grant Agency of the Ministry of Health of the Czech Republic [NT12156]
- Ministry of Education of the Czech Republic [ME 10145]
- CENTEM project [CZ.1.05/2.1.00/03.0088]
- ERDF as part of the Ministry of Education, Youth and Sports
- European Centre of Excellence [CZ.1.05/1.1.00/02.0090]
Objectives We prepared 3D poly (e-caprolactone) (PCL) nanofibre scaffolds and tested their use for seeding, proliferation, differentiation and migration of mesenchymal stem cell (MSCs). Materials and methods 3D nanofibres were prepared using a special collector for common electrospinning; simultaneously, a 2D PCL nanofibre layer was prepared using a classic plain collector. Both scaffolds were seeded with MSCs and biologically tested. MSC adhesion, migration, proliferation and osteogenic differentiation were investigated. Results The 3D PCL scaffold was characterized by having better biomechanical properties, namely greater elasticity and resistance against stress and strain, thus this scaffold will be able to find broad applications in tissue engineering. Clearly, while nanofibre layers of the 2D scaffold prevented MSCs from migrating through the conformation, cells infiltrated freely through the 3D scaffold. MSC adhesion to the 3D nanofibre PCL layer was also statistically more common than to the 2D scaffold (P < 0.05), and proliferation and viability of MSCs 2 or 3 weeks post-seeding, were also greater on the 3D scaffold. In addition, the 3D PCL scaffold was also characterized by displaying enhanced MSC osteogenic differentiation. Conclusions We draw the conclusion that all positive effects observed using the 3D PCL nanofibre scaffold are related to the larger fibre surface area available to the cells. Thus, the proposed 3D structure of the nanofibre layer will find a wide array of applications in tissue engineering and regenerative medicine.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据