期刊
CELL PROLIFERATION
卷 44, 期 2, 页码 183-191出版社
WILEY
DOI: 10.1111/j.1365-2184.2011.00737.x
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资金
- Academy of Sciences of the Czech Republic [AV0Z50390703, AV0Z50390512]
- Ministry of Education, Youth and Sports of the Czech Republic [NPV II 2B06130, 1M0510, P304/10/1307]
- Grant Agency of the Academy of Sciences [IAA500390702]
- Czech Science Foundation [GA202/09/1151]
- EU [7E09088]
- Grant Agency of the Charles University [119009, 96610, 119209, 97110, 80009]
Objectives: The aim of this study was to develop functionalized nanofibres as a simple delivery system for growth factors (GFs) and make nanofibre cell-seeded scaffold implants a one-step intervention. Materials and methods: We have functionalized polycaprolactone (PCL) nanofibres with thrombocytes adherent on them. Immobilized, these thrombocytes attached to nanofibre scaffolds were used as a nanoscale delivery system for native (autologous) proliferation and differentiation factors, in vitro. Pig chondrocytes were seeded on the thrombocyte-coated scaffolds and levels of proliferation and differentiation of these cells were compared with those seeded on non-coated scaffolds. Results: Immobilized thrombocytes on PCL nanofibres effectively enhanced chondrocyte proliferation due to time-dependent degradation of thrombocytes and release of their GFs. Conclusions: These simply functionalized scaffolds present new possibilities for nanofibre applications, as smart cell scaffolds equipped with a GF delivery tool.
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