期刊
JOURNAL OF FLUORESCENCE
卷 13, 期 1, 页码 79-88出版社
KLUWER ACADEMIC/PLENUM PUBL
DOI: 10.1023/A:1022358614995
关键词
coproporphyrin; monoclonal antibodies; reverse micelles; aerosol OT; fluorescence quenching
The antigen-antibody interaction between coproporphyrin I and anti-coproporphyrin antibodies was studied by a fluorescence method in water and a reverse micellar system: n-octane/Aerosol OT. Coproporphyrin fluorescence was quenched, and coproporphyrin emission maximum was shifted to the long-wavelength region after binding to the antibodies or Fab'-fragments. The mechanism of this quenching is static, most probably, by a tryptophan residue (or maybe lysine or methionine). Apparent dynamic quenching, in this case, arises from protein backbone motion. A special kind of antibody Fab'-Fab' dimerization was proposed.
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