期刊
CELL METABOLISM
卷 7, 期 1, 页码 86-94出版社
CELL PRESS
DOI: 10.1016/j.cmet.2007.11.002
关键词
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资金
- NHGRI NIH HHS [HG01696, R01 HG001696, R01 HG001696-03] Funding Source: Medline
- NIDDK NIH HHS [R01 DK63996, R01 DK063906-05, R01 DK063906, R21 DK063996] Funding Source: Medline
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [R01HG001696] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R21DK063996, R01DK063906] Funding Source: NIH RePORTER
We have sought to identify transcriptional pathways in adipogenesis using an integrated experimental and computational approach. Here, we employ high-throughput DNase hypersensitivity analysis to find regions of altered chromatin structure surrounding key adipocyte genes. Regions that display differentiation-dependent changes in hypersensitivity were used to predict binding sites for proteins involved in adipogenesis. A high-scoring example was a binding motif for interferon regulatory factor (IRF) family members. Expression of all nine mammalian IRF mRNAs is regulated during adipogenesis, and several bind to the identified motifs in a differentiation-dependent manner. Furthermore, several IRF proteins repress differentiation. This analysis suggests an important role for IRF proteins in adipocyte biology and demonstrates the utility of this approach in identifying cis- and trans-acting factors not previously suspected to participate in adipogenesis.
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