期刊
CELL HOST & MICROBE
卷 15, 期 5, 页码 636-643出版社
CELL PRESS
DOI: 10.1016/j.chom.2014.04.007
关键词
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资金
- UCSF Nikon Imaging Center
- EMBO
- NIH [RO1 AI065902, PO1 AI53194, K99CA163535, K12 HD072222, K12 HD000850, K08 DK068358]
The mucosal epithelium consists of polarized cells with distinct apical and basolateral membranes that serve as functional and physical barriers to external pathogens. The apical surface of the epithelium constitutes the first point of contact between mucosal pathogens, such as Pseudomonas aeruginosa, and their host. We observed that binding of P. aeruginosa aggregates to the apical surface of polarized cells led to the striking formation of an actin-rich membrane protrusion with inverted polarity, containing basolateral lipids and membrane components. Such protrusions were associated with a spatially localized host immune response to P. aeruginosa aggregates that required bacterial flagella and a type III secretion system apparatus. Host protrusions formed de novo underneath bacterial aggregates and involved the apical recruitment of a Par3/Par6a/aPKC/Rac1 signaling module for a robust, spatially localized host NF-kB response. Our data reveal a role for spatiotemporal epithelial polarity changes in the activationof innate immune responses.
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