期刊
CELL HOST & MICROBE
卷 14, 期 6, 页码 683-695出版社
CELL PRESS
DOI: 10.1016/j.chom.2013.11.010
关键词
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资金
- Science Foundation Ireland [08/IN.1/B2104]
- University of Liverpool
- Science Foundation Ireland (SFI) [08/IN.1/B2104] Funding Source: Science Foundation Ireland (SFI)
Bacterial transcriptional networks consist of hundreds of transcription factors and thousands of promoters. However, the true complexity of transcription in a bacterial pathogen and the effect of the environments encountered during infection remain to be established. We present a simplified approach for global promoter identification in bacteria using RNA-seq-based transcriptomic analyses of 22 distinct infection-relevant environmental conditions. Individual RNA samples were combined to identify most of the 3,838 Salmonella enterica serovar Typhimurium promoters in just two RNA-seq runs. Individual in vitro conditions stimulated characteristic transcriptional signatures, and the suite of 22 conditions induced transcription of 86% of all S. Typhimurium genes. We highlight the environmental conditions that induce the Salmonella pathogenicity islands and present a small RNA expression landscape of 280 sRNAs. This publicly available compendium of environmentally controlled expression of every transcriptional feature of S. Typhimurium constitutes a useful resource for the bacterial research community.
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