期刊
CELL HOST & MICROBE
卷 7, 期 4, 页码 324-334出版社
CELL PRESS
DOI: 10.1016/j.chom.2010.03.008
关键词
-
资金
- German Bundesministerium fuer Bildung und Forschung [01GS0801/3/4]
- MAC [G0501453]
- Friednch-Baur Stiftung
- CNRS
- Ligue Contre le Cancer
- Deutsche Forschungsgemeinschaft [FO855, SFB 576]
- Max-Planck-Society
- Bayerisches Staatsministerium fur Wissenschaft, Forschung und Kunst (BayGene)
- MRC [G0501453] Funding Source: UKRI
- Medical Research Council [G0501453] Funding Source: researchfish
The mRNA targets of microRNAs (miRNAs) can be identified by immunoprecipitation of Argonaute (Ago) protein-containing RNA-induced silencing complexes (RISCs) followed by microarray analysis (RIP-Chip). Here we used Ago2-based RIP-Chip to identify transcripts targeted by Kaposi's sarcoma-associated herpesvirus (KSHV) miRNAs (n = 114), Epstein-Barr virus (EBV) miRNAs (n = 44), and cellular miRNAs (n = 2337) in six latently infected or stably transduced human B cell lines. Of the six KSHV miRNA targets chosen for validation, four showed regulation via their 3'UTR, while two showed regulation via binding sites within coding sequences. Two genes governing cellular transport processes (TOMM22 and IPO7) were confirmed to be targeted by EBV miRNAs. A significant number of viral miRNA targets were upregulated in infected cells, suggesting that viral miRNAs preferentially target cellular genes induced upon infection. Transcript half-life both of cellular and viral miRNA targets negatively correlated with recruitment to RISC complexes, indicating that RIP-Chip offers a quantitative estimate of miRNA function.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据