期刊
CELL HOST & MICROBE
卷 7, 期 1, 页码 74-81出版社
CELL PRESS
DOI: 10.1016/j.chom.2009.12.009
关键词
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资金
- NSF [IOB 0517218]
- NIH-NIAID [5R21A1072170]
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [0817790] Funding Source: National Science Foundation
Viruses change rapidly due to genetic mutations, and viral RNA recombination in RNA viruses can lead to the emergence of drug-resistant or highly virulent strains. Here, we report that host Pmr1p, an ion pump that controls Ca2+/Mn2+ influx into the Golgi from the cytosol, affects the frequency of viral RNA recombination and the efficiency of replication. Inactivation of PMR1 leads to an similar to 160-fold increase in RNA recombination of Tomato bushy stunt virus (TBSV) in yeast, a model host. Expression of separation-of-function mutants of Pmr1p reveals that the ability of Pmr1p to control the Mn2+ concentration in the cytosol is a key factor in viral RNA recombination. Indeed, a high Mn2+ concentration in a cell-free TBSV replication system increases the recombination frequency, and knockdown of Ca2+/Mn2+ exporters in plants increases virus replication and RNA recombination. Thus, a conserved host protein could affect the adaptive evolution of RNA viruses.
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