期刊
CELL HOST & MICROBE
卷 6, 期 6, 页码 551-562出版社
CELL PRESS
DOI: 10.1016/j.chom.2009.11.007
关键词
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资金
- German Federal Ministry for Education and Research (BMBF-BioFuture)
- German Research Foundation [SFB 544]
- Frontier Program of the University of Heidelberg
- Institute for Computational Modeling
- Siberian Branch of the Russian Academy of Science
- Krasnoyarsk
- Center for Modelling and Simulation (BIOMS)
- Cluster of Excellence CellNetworks
- Medical School at the University of Heidelberg
- Max Planck Society
- Karlsruhe Institute of Technology (KIT)
- European Network of Excellence BioMalPar
Sporozoites are the highly motile stages of the malaria parasite injected into the host's skin during a mosquito bite. In order to navigate inside of the host, sporozoites rely on actin-dependent gliding motility. Although the major components of the gliding machinery are known, the spatiotemporal dynamics of the proteins and the underlying mechanism powering forward locomotion remain unclear. Here, we show that sporozoite motility is characterized by a continuous sequence of stick-and-slip phases. Reflection interference contrast and traction force microscopy identified the repeated turnover of discrete adhesion sites as the underlying mechanism of this substrate-dependent type of motility. Transient forces correlated with the formation and rupture of distinct substrate contact sites and were dependent on actin dynamics. Further, we show that the essential sporozoite surface protein TRAP is critical for the regulated formation and rupture of adhesion sites but is dispensable for retrograde capping.
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