期刊
FISH PATHOLOGY
卷 38, 期 4, 页码 171-176出版社
JAPAN SOC FISH PATHOL DEPT FISHERIES-FAC AGR
DOI: 10.3147/jsfp.38.171
关键词
adjuvant; vaccine; bacterial coldwater disease; Flavobacterium psychrophilum; toxicity; Plecoglossus altivelis; ayu
Bacterial coldwater disease is considered to be one of the most serious diseases in ayu Plecoglossus altivelis in Japan. We evaluated the efficacy of three highly water-soluble adjuvants (Montanidae IMS-1311, IMS-1312 and IMS-2212) combined with formalin-killed bacterin of Flavobacterium psychrophilum (FKB) in ayu, in comparison with a minimally water-soluble adjuvant (Montanidae ISA-763A). The retention period and toxicity of these adjuvants in ayu were also examined. Experimental challenge 4 wk after vaccination showed that FKB adjuvanted with IMS-1311 and IMS-1312 had higher potency than FKB alone and significantly (p < 0.05) higher potency than the control with sterile distilled water. Residues of the highly water-soluble adjuvants in ayu remained for shorter periods than that of the minimally water-soluble adjuvant, although the former adjuvants were acutely toxic at doses higher than 25 muL/fish(1.7 +/- 0.5 g). The high level of protection, short retention period and lack of toxicity indicate that a dose of 5 muL of IMS-1311 or IMS-1312 combined with 5 muL of FKB makes a promising vaccine against bacterial coldwater disease in ayu.
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