期刊
CELL DEATH AND DIFFERENTIATION
卷 22, 期 7, 页码 1106-1116出版社
SPRINGERNATURE
DOI: 10.1038/cdd.2014.181
关键词
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资金
- British Heart Foundation studentship
- British Heart Foundation Fellowship Transition Award
- Medical Research Council studentship
- Early Career Researcher grant from the Society for Endocrinology
- British Heart Foundation Centre of Research Excellence award
- Medical Research Council [1202042] Funding Source: researchfish
Glucocorticoid levels rise dramatically in late gestation to mature foetal organs in readiness for postnatal life. Immature heart function may compromise survival. Cardiomyocyte glucocorticoid receptor (GR) is required for the structural and functional maturation of the foetal heart in vivo, yet the molecular mechanisms are largely unknown. Here we asked if GR activation in foetal cardiomyocytes in vitro elicits similar maturational changes. We show that physiologically relevant glucocorticoid levels improve contractility of primary-mouse-foetal cardiomyocytes, promote Z-disc assembly and the appearance of mature myofibrils, and increase mitochondrial activity. Genes induced in vitro mimic those induced in vivo and include PGC-1 alpha, a critical regulator of cardiac mitochondrial capacity. SiRNA-mediated abrogation of the glucocorticoid induction of PGC-1 alpha in vitro abolished the effect of glucocorticoid on myofibril structure and mitochondrial oxygen consumption. Using RNA sequencing we identified a number of transcriptional regulators, including PGC-1 alpha, induced as primary targets of GR in foetal cardiomyocytes. These data demonstrate that PGC-1 alpha is a key mediator of glucocorticoid-induced maturation of foetal cardiomyocyte structure and identify other candidate transcriptional regulators that may play critical roles in the transition of the foetal to neonatal heart.
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