期刊
CELL DEATH AND DIFFERENTIATION
卷 21, 期 1, 页码 69-78出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/cdd.2013.72
关键词
TLR4; immunogenic cell death; chemotherapy; Dendrophilin; cancer
资金
- Innaxon, UK
- Institut National du Cancer (INCa)
- la Ligue contre le cancer (LIGUE labellisee, Zitvogel L, LIGUE (NP, AS)
- l'Association pour la Recherche sur le Cancer (ARC)
- Fondation pour la Recherche Medicale and Fondation de France, SIRIC, PACRI, LABEX ImmunoONcology
- European Research Council (ERC)
- European Commission (ArtForce)
- Agence National de la Recherche (ANR)
- Ligue contre le Cancer (Equipe labellisee)
- Fondation pour la Recherche Medicale (FRM)
- LabEx Immuno-Oncologie
- Fondation de France
- Fondation Bettencourt-Schueller
- AXA Chair for Longevity Research
- Canceropole Ile-de-France
- Paris Alliance of Cancer Research Institutes (PACRI)
Immunogenic cell death induced by anticancer chemotherapy is characterized by a series of molecular hallmarks that include the exodus of high-mobility group box 1 protein (HMGB1) from dying cells. HMGB1 is a nuclear nonhistone chromatin-binding protein. It is secreted at the late stages of cellular demise and engages Toll-like receptor4 (TLR4) on dendritic cells (DCs) to accelerate the processing of phagocytic cargo in the DC and to facilitate antigen presentation by DC to T cells. The absence of HMGB1 expression by dying tumor cells exposed to anthracyclines or oxaliplatin compromises DC-dependent T-cell priming by tumor-associated antigens. Here, we show that transplantable tumors exhibiting weak expression of nuclear HMGB1 respond to chemotherapy more effectively if the treatment is combined with the local or systemic administration of a highly purified and physiochemically defined and standardized lipopolysaccharide solution, which acts as a high-potency and exclusive TLR4 agonist, called Dendrophilin (DEN). The synergistic antitumor effects mediated by the combination of chemotherapy and immunotherapy relied upon the presence of the MyD88 (myeloid differentiation primary response gene) adapter of TLR4 (but not that of the TIR-domain-containing adapter-inducing interferon-beta adapter), in line with the well-characterized action of DEN on the MyD88 signaling pathway. DEN and anthracyclines synergized to induce intratumoral accumulation of interferon-c-producing CD4(+) and CD8(+) T lymphocytes. Moreover, DEN could restore the immunogenicity of dying tumor cells from which HMGB1 had been depleted by RNA interference. These findings underscore the potential clinical utility of combination regimens involving immunogenic chemotherapy and certain TLR4 agonists in advanced HMGB1-deficient cancers.
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