期刊
CELL DEATH AND DIFFERENTIATION
卷 19, 期 9, 页码 1525-1535出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/cdd.2012.30
关键词
tumor suppressor; autophagy; p53; LC3; p62
资金
- Institut National de la Sante et de la Recherche Medicale (INSERM)
- Centre National de la Recherche Scientifique
- Institut National du Cancer
- Canceropole PACA
- La Ligue Nationale Contre le Cancer
- Association pour la Recherche sur le Cancer
- Agence Nationale de la Recherche
- ECOS SUD programme
- Ministere de la Recherche et de la Technologie
TP53INP1 (tumor protein 53-induced nuclear protein 1) is a tumor suppressor, whose expression is downregulated in cancers from different organs. It was described as a p53 target gene involved in cell death, cell-cycle arrest and cellular migration. In this work, we show that TP53INP1 is also able to interact with ATG8-family proteins and to induce autophagy-dependent cell death. In agreement with this finding, we observe that TP53INP1, which is mainly nuclear, relocalizes in autophagosomes during autophagy where it is eventually degraded. TP53INP1-LC3 interaction occurs via a functional LC3-interacting region (LIR). Inactivating mutations of this sequence abolish TP53INP1-LC3 interaction, relocalize TP53INP1 in autophagosomes and decrease TP53INP1 ability to trigger cell death. Interestingly, TP53INP1 binds to ATG8-family proteins with higher affinity than p62, suggesting that it could partially displace p62 from autophagosomes, modifying thereby their composition. Moreover, silencing the expression of autophagy related genes (ATG5 or Beclin-1) or inhibiting caspase activity significantly decreases cell death induced by TP53INP1. These data indicate that cell death observed after TP53INP1-LC3 interaction depends on both autophagy and caspase activity. We conclude that TP53INP1 could act as a tumor suppressor by inducing cell death by caspase-dependent autophagy. Cell Death and Differentiation (2012) 19, 1525-1535; doi:10.1038/cdd.2012.30; published online 16 March 2012
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