Specific primers for the differentiation of Heterobasidion annosum (s.str.) and H-parviporum infected stumps in northern Europe

Two separate amplification products from random amplified microsatellite fingerprints of Heterobasidion annosum (s.str.) and H. parviporum were converted to specific markers. The markers were tested to be species specific and combined to a single PCR-reaction, which allowed the detection and identification of the two fungi directly from wood samples.