期刊
CELL DEATH AND DIFFERENTIATION
卷 16, 期 4, 页码 613-623出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/cdd.2008.187
关键词
apoptosis; cytochrome-c; caspases; mitochondrial outer membrane permeabilization; diffusion modeling
资金
- Science Foundation Ireland [03/RP1/B344, 05/RFP/BIM056]
- Health Research Board Ireland [RP/2006/258]
- National Biophotonics and Imaging Platform
- EU Framework Programme 7
- Science Foundation Ireland (SFI) [03/RP1/B344] Funding Source: Science Foundation Ireland (SFI)
Individual cells within a population undergo apoptosis at distinct, apparently random time points. By analyzing cellular mitotic history, we identified that sibling HeLa cell pairs, in contrast to random cell pairs, underwent apoptosis synchronously. This allowed us to use high-speed cellular imaging to investigate mitochondrial outer membrane permeabilization (MOMP), a highly coordinated, rapid process during apoptosis, at a temporal resolution approximately 100 times higher than possible previously. We obtained new functional and mechanistic insight into the process of MOMP: We were able to determine the kinetics of pore formation in the outer mitochondrial membrane from the initiation phase of cytochrome-c-GFP redistribution, and showed differential pore formation kinetics in response to intrinsic or extrinsic apoptotic stimuli (staurosporine, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)). We also detected that the onset of mitochondrial permeabilization frequently proceeded as a wave through the cytosol, and that the frequency of wave occurrence in response to TRAIL was reduced by inhibition of protein kinase CK2. Computational analysis by a partial differential equation model suggested that the spread of permeabilization signals could sufficiently be explained by diffusion-adsorption velocities of locally generated permeabilization inducers. Taken together, our study yielded the first comprehensive analysis of clonal cell-to-cell variability in apoptosis execution and allowed to visualize and explain the dynamics of MOMP in cells undergoing apoptosis.
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