期刊
CELL CYCLE
卷 13, 期 13, 页码 2064-2072出版社
LANDES BIOSCIENCE
DOI: 10.4161/cc.29083
关键词
BI2536; Polo kinase; centrosome maturation; spindle assembly; centriole disengagement; gametogenesis; Drosophila
类别
资金
- PRIN
- Cancer Research UK [11431] Funding Source: researchfish
Pharmacological inhibition of Drosophila Polo kinase with BI2536 has allowed us to re-examine the requirements for Polo during Drosophila male gametogenesis. BI2536-treated spermatocytes persisted in a pro-metaphase state without dividing and had condensed chromosomes that did not separate. Centrosomes failed to recruit.-tubulin and centrosomin (Cnn) and were not associated with microtubule arrays that were abnormal and did not form proper bipolar spindles. Centrioles, which usually separate during the anaphase of the first meiosis, remained held together in a V-shaped configuration suggesting that Polo kinase regulates the proteolysis that breaks centriole linkage to ensure their disengagement. Despite these defects spermatid differentiation proceeds, leading to axoneme formation.
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