期刊
CELL CYCLE
卷 13, 期 20, 页码 3241-3248出版社
LANDES BIOSCIENCE
DOI: 10.4161/15384101.2014.953876
关键词
Cell cycle; Replication; Replication timing; S-phase; Transcription
类别
资金
- Swedish Cancer Research foundation (Cancerfonden: Kontrakt) [100422]
- Swedish Research Council [VR-M: K2014-67X-20781-07-4]
- Barncancerfonden [PR2013-0048]
- LUA/ALF
Transcriptional events during S-phase are critical for cell cycle progression. Here, by using a nascent RNA capture assay coupled with high-throughput sequencing, we determined the temporal patterns of transcriptional events that occur during S-phase. We show that genes involved in critical S-phase-specific biological processes such as nucleosome assembly and DNA repair have temporal transcription patterns across S-phase that are not evident from total RNA levels. By comparing transcription timing with replication timing in S-phase, we show that early replicating genes show increased transcription late in S-phase whereas late replicating genes are predominantly transcribed early in S-phase. Global anti-correlation between replication and transcription timing was observed only based on nascent RNA but not total RNA. Our data provides a detailed view of ongoing transcriptional events during the S-phase of cell cycle, and supports that transcription and replication are temporally separated.
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