期刊
CELL CYCLE
卷 12, 期 19, 页码 3194-3202出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/cc.26241
关键词
RNA-binding proteins; cytoplasmic RNP complexes; protein aggregation; proteinopathy; FUSopathy; amyotrophic lateral sclerosis
类别
资金
- Welcome Trust [075615/Z/04/z]
- Short-Term EMBO Fellowship [ASTF 351-2011]
- Russian Foundation for Basic Research_mol_a Grant [12-04-31791]
- Cardiff NMHRI 4-y PhD Studentship Programme
Fused in sarcoma (FUS) belongs to the group of RNA-binding proteins implicated as underlying factors in amyotrophic lateral sclerosis (ALS) and certain other neurodegenerative diseases. Multiple FUS gene mutations have been linked to hereditary forms, and aggregation of FUS protein is believed to play an important role in pathogenesis of these diseases. In cultured cells, FUS variants with disease-associated amino acid substitutions or short deletions affecting nuclear localization signal (NLS) and causing cytoplasmic mislocalization can be sequestered into stress granules (SGs). We demonstrated that disruption of motifs responsible for RNA recognition and binding not only prevents SG recruitment, but also dramatically increases the protein propensity to aggregate in the cell cytoplasm with formation of juxtanuclear structures displaying typical features of aggresomes. Functional RNA-binding domains from TAR DNA-binding protein of 43 kDa (TDP-43) fused to highly aggregation-prone C-terminally truncated FUS protein restored the ability to enter SGs and prevented aggregation of the chimeric protein. Truncated FUS was also able to trap endogenous FUS molecules in the cytoplasmic aggregates. Our data indicate that RNA binding and recruitment to SGs protect cytoplasmic FUS from aggregation, and loss of this protection may trigger its pathological aggregation in vivo.
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