4.6 Article Retracted Publication

被撤回的出版物: Polypyrimidine tract-binding protein regulates the cell cycle through IRES-dependent translation of CDK11p58 in mouse embryonic stem cells (Retracted article. See vol. 15, pg. 2992, 2016)

期刊

CELL CYCLE
卷 10, 期 21, 页码 3706-3713

出版社

TAYLOR & FRANCIS INC
DOI: 10.4161/cc.10.21.17903

关键词

polypyrimidine tract-binding protein; PTB; embryonic stem cells; M phase; IRES-dependent translation; CDK11(p58); PITSLRE

资金

  1. MEXT, Japan
  2. Grants-in-Aid for Scientific Research [22500286, 23790218, 22500384] Funding Source: KAKEN

向作者/读者索取更多资源

Polypyrimidine tract-binding protein (PTB/PTBP1/hnRNP I) is a member of the heterogeneous nuclear ribonucleoprotein family that binds specifically to pyrimidine-rich sequences of RNAs. Although PTB is a multifunctional protein involved in RNA processing and internal ribosome entry site (IRE S)-dependent translation, the role of PTB in early mouse development is unclear. Ptb knockout mice exhibit embryonic lethality shortly after implantation and Ptb(-/-) embryonic stem (ES) cells have a severe proliferation defect that includes a prolonged G(2)/M phase. The present study shows that PTB promotes M phase progression by the direct repression of CDK11(p58) IRE S activity in ES cells. The protein expression and IRE S activity of CDK11(p58) in Ptb(-/-) ES cells is higher than that of wild-type ES cells, indicating that PTB is involved in the repression of CDK11(p58) expression through IRE S-dependent translation in ES cells. Interestingly, CDK11(p58) IRE S activity is activated by upstream of N-Ras (UNR) in 293T and NIH3T3 cells, whereas UNR is not present in the Cdk11 mRNA-protein complex in ES cells. In addition, PTB interacts directly with the IRE S region of CDK11(p58) in ES cells. These results suggest that PTB regulates the precise expression of CDK11(p58) through direct interaction with CDK11(p58) IRE S and promotes M phase progression in ES cells.

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