期刊
CELL CYCLE
卷 9, 期 13, 页码 2581-2588出版社
LANDES BIOSCIENCE
DOI: 10.4161/cc.9.13.12076
关键词
Cin8; cluster; GFP-tubulin; kinesin-5; kinesin-8; kinetochore; Kip3; metaphase; microtubule; mitosis; spindle
类别
资金
- PHS [R01GM40506, R01GM69429]
- National Institute of General Medical Sciences [2T32 GM007270]
- NCRR [P41RR011823]
- Searle Scholar Award [06-L-111]
- Packard Fellowship for Science and Engineering [2006-30521]
In Saccharomyces cerevisiae, chromosome congression clusters kinetochores on either side of the spindle equator at metaphase. Many organisms require one or more kinesin-8 molecular motors to achieve chromosome alignment. The yeast kinesin-8, Kip3, has been well studied in vitro but a role in chromosome congression has not been reported. We investigated Kip3's role in this process using semi-automated, quantitative fluorescence microscopy and time-lapse imaging and found that Kip3 is required for congression. Deletion of KIP3 increases inter-kinetochore distances and increases the variability in the position of sister kinetochores along the spindle axis during metaphase. Kip3 does not regulate spindle length and is not required for kinetochore-microtubule attachment. Instead, Kip3 clusters kinetochores on the metaphase spindle by tightly regulating kinetochore microtubule lengths.
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