期刊
CELL CYCLE
卷 9, 期 7, 页码 1360-1372出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/cc.9.7.11177
关键词
cell cycle; mitosis; chromosomal passenger complex; chromosome alignment
类别
资金
- Deutsche Forschungsgemeinschaft
During mitosis, the chromosomal passenger complex (CpC) comprising the Aurora B kinase, INCeNp, survivin and borealin is essential for correcting non-bipolar chromosome attachments and for cytokinesis. In addition, the CpC might fullfil a role in the mitotic spindle assembly checkpoint (SAC), but this activity might be related to its role in correcting non-bipolar chromosome attachments. Here, we demonstrate that treatment of mitotic cells with the antibiotic actinomycin D causes a displacement of an intact and active CpC from centromeres onto chromosome arms, which results in chromosome misalignment, cytokinesis failure and SAC override, but still preserves histone H3 phosphorylation on chromosome arms. this surprising and unique scenario allows the reconstitution of endogenous Aurora B at centromeres/inner kinetochores by expressing a Cenp-B-INCeNp fusion protein. We find that although the selective recruitment of endogenous Aurora B to centromeres/inner kinetochores is not sufficient to restore chromosome alignment and cytokinesis, it can restore Cenp-A phosphorylation at kinetochores, BubR1 recruitment to kinetochores and SAC activity after spindle disruption. these results indicate that INCeNp-Aurora B localized at centromeres/inner kinetochores is sufficient to mediate SAC activity upon spindle disruption.
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