期刊
NUCLEIC ACIDS RESEARCH
卷 31, 期 23, 页码 6741-6747出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkg909
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资金
- NCI NIH HHS [R01CA84890, R01 CA084890] Funding Source: Medline
- NATIONAL CANCER INSTITUTE [R01CA084890] Funding Source: NIH RePORTER
The methylation of histone H3 correlates with either gene expression or silencing depending on the residues modified. Methylated lysine 4 (H3-K4) is associated with transcription at active gene loci. Furthermore, it was reported that trimethylated but not dimethylated H3-K4 is exclusively associated with active chromatin in Saccharomyces cerevisiae. In the present study, we investigated the H3-K4 methylation at the human prostate specific antigen (PSA) locus following gene activation and repression via androgen receptor (AR). We show that ligand-induced, AR-mediated transcription was accompanied by rapid decreases in di- and trimethylated H3-K4 at the PSA enhancer and promoter. Moreover, the observed decreases in H3-K4 methylation were reversed when AR was inhibited by a specific AR antagonist, bicalutamide. In contrast to the decreases in methylation at the 5' transcriptional control regions of the PSA gene, H3-K4 methylation in the coding region steadily increased after a lag period of similar to4 h. The results suggest a novel role of methylated H3-K4 in transcriptional regulation.
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