期刊
CELL CYCLE
卷 8, 期 3, 页码 454-459出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/cc.8.3.7649
关键词
HOXC13; lamin B2; TOP1; MCM4; replication foci; GFP fusion; immuno-fluorescence
类别
资金
- Associazione Italiana per la Ricerca sul Cancro
- Fondazione Monte dei Paschi di Siena
- Istituto Toscano Tumori
- Fondazione Cariplo-Progetto Nobel
The homeotic (and oncogenic) HOXC13 protein was shown to have an affinity for a DNA fragment corresponding to the sequence covered by the pre-replicative complex of the human lamin B2 replication origin. We show here that HOXC13 is a member of human replicative complexes. Our fluorescent fusion-protein data demonstrate that it co-localizes with replication foci of early-S cells and that this peculiar behaviour is driven by the homeodomain. By ChIP analysis we also show that HOXC13 binds the lamin B2 replication origin and the origins located near the TOP1 and MCM4 genes in asynchronously growing cells, whereas it does not bind these origins in G(0) resting cells, consistently with its involvement in origin function.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据