期刊
CELL COMMUNICATION AND ADHESION
卷 15, 期 3, 页码 289-303出版社
INFORMA HEALTHCARE
DOI: 10.1080/15419060802198736
关键词
cardiac fibroblast; connexin; Cx43; Cx45; murine; proliferation
资金
- American Heart Association
- Canadian Institutes for Health Research
- Heart and Stroke Foundation of Canada
- NIH/NHLBI [HL-066350]
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL066350] Funding Source: NIH RePORTER
Little is known about connexin expression and function in murine cardiac fibroblasts. The authors isolated native ventricular fibroblasts from adult mice and determined that although they expressed both connexin43 (Cx43) and connexin45 (Cx45), the relative abundance of Cx45 was greater than that of Cx43 in fibroblasts compared to myocytes, and the electrophoretic mobility of both Cx43 and Cx45 differed in fibroblasts and in myocytes. Increasing Cx43 expression by adenoviral infection increased intercellular coupling, whereas decreasing Cx43 expression by genetic ablation decreased coupling. Interestingly, increasing Cx43 expression reduced fibroblast proliferation, whereas decreasing Cx43 expression increased proliferation. These data demonstrate that native fibroblasts isolated from the mouse heart exhibit intercellular coupling via gap junctions containing both Cx43 and Cx45. Fibroblast proliferation is inversely related to the expression level of Cx43. Thus, connexin expression and remodeling is likely to alter fibroblast function, maintenance of the extracellular matrix, and ventricular remodeling in both normal and diseased hearts.
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