期刊
JOURNAL OF INVESTIGATIVE DERMATOLOGY
卷 121, 期 5, 页码 963-968出版社
BLACKWELL PUBLISHING INC
DOI: 10.1046/j.1523-1747.2003.12600.x
关键词
transgenic; wound healing; alopecia; aging; carcinogenesis
类别
资金
- NIAMS NIH HHS [R29-AR-44038, R01-AR46837] Funding Source: Medline
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [R01AR046837, R29AR044038] Funding Source: NIH RePORTER
Putative epithelial stem cells in the hair follicle bulge are thought to play pivotal roles in the homeostasis, aging, and carcinogenesis of the cutaneous epithelium. Elucidating the role of bulge cells in these processes has been hampered by the lack of gene promoters that target this area with specificity. Here we describe the isolation of the mouse keratin 15 (K15) promoter and demonstrate its utility for preferentially targeting hair follicle bulge cells in adult K15/lacZ transgenic mice. We found that patterns of K15 expression and promoter activity changed with age and correlated with levels of differentiation within the cutaneous epithelium; less differentiated keratinocytes in the epidermis of the neonatal mouse and in the bulge area of the adult mouse preferentially expressed K15. These findings demonstrate the utility of the K15 promoter for targeting epithelial stem cells in the hair follicle bulge and set the stage for elucidating the role of bulge cells in skin biology.
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