期刊
CELL CALCIUM
卷 54, 期 6, 页码 387-394出版社
CHURCHILL LIVINGSTONE
DOI: 10.1016/j.ceca.2013.09.001
关键词
Awake; Astrocyte; Norepinephrine; Calcium; Startle
类别
资金
- US National Institutes of Health [NS075177, NS078304]
- Research Council of Norway
- Nordic Center of Excellence Program
- Letten Foundation
- Fulbright Foundation
Astrocyte Ca2+ signals in awake behaving mice are widespread, coordinated and differ fundamentally from the locally restricted Ca2+ transients observed ex vivo and in anesthetized animals. Here we show that the synchronized release of norepinephrine (NE) from locus coeruleus (LC) projections throughout the cerebral cortex mediate long-ranging Ca2+ signals by activation of astrocytic alpha(1)-adrenergic receptors. When LC output was triggered by either physiological sensory (whisker) stimulation or an air-puff startle response, astrocytes responded with fast Ca2+ transients that encompassed the entire imaged field (positioned over either frontal or parietal cortex). The application of adrenergic inhibitors, including alpha(1)-adrenergic antagonist prazosin, potently suppressed both evoked, as well as the frequently observed spontaneous astroglial Ca2+ signals. The LC-specific neurotoxin N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP-4), which reduced cortical NE content by >90%, prevented nearly all astrocytic Ca2+ signals in awake mice. The observations indicate that in adult, unanesthetized mice, astrocytes do not respond directly to glutamatergic signaling evoked by sensory stimulation. Instead astrocytes appear to be the primary target for NE, with astrocytic Ca2+ signaling being triggered by the alpha(1)-adrenergic receptor. In turn, astrocytes may coordinate the broad effects of neuromodulators on neuronal activity. (C) 2013 Elsevier Ltd. All rights reserved.
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