期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 69, 期 11, 页码 6698-6702出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.69.11.6698-6702.2003
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资金
- NIAID NIH HHS [AI 42708] Funding Source: Medline
- NIGMS NIH HHS [R01 GM067937, GM067937] Funding Source: Medline
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM067937] Funding Source: NIH RePORTER
The medicinal value associated with complex polyketide and nonribosomal peptide natural products has prompted biosynthetic schemes dependent upon heterologous microbial hosts. Here we report the successful biosynthesis of yersiniabactin (Ybt), a model polyketide-nonribosomal peptide hybrid natural product, using Escherichia coli as a heterologous host. After introducing the biochemical pathway for Ybt into E. coli, biosynthesis was initially monitored qualitatively by mass spectrometry. Next, production of Ybt was quantified in a high-cell-density fermentation environment with titers reaching 67 +/- 21 (mean standard deviation) mg/liter and a volumetric productivity of 1.1 +/- 0.3 mg/liter-h. This success has implications for basic and applied studies on Ybt biosynthesis and also, more generally, for future production of polyketide, nonribosomal peptide, and mixed polyketide-nonribosomal peptide natural products using E. coli.
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