期刊
PLANT AND CELL PHYSIOLOGY
卷 44, 期 11, 页码 1168-1175出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcg142
关键词
developing seed; embryo; lipoxygenase (LOX); linolenic acid; rice
We isolated a novel C9 position specific lipoxygenase (r9-LOX1) cDNA from developing rice seeds. The enzymatic features of r9-LOX1 resembled those of rice LOX-L3 known to be contained in rice germ and to have C9-specific LOX activity. However, the expression level of the r9-LOX1 gene was higher in imbibed seeds rather than developing seeds. A homology search against the rice nucleotide database revealed the r9-LOX1 gene to be on rice chromosome 3 (accession number AC093017). The restriction enzyme map of the reported genomic sequence agreed with the result of the Southern blot analysis for the r9-LOX1. The enzyme could be useful for in vitro synthesis of 9,10-ketol-octadecadienoic acid.
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