期刊
CELL CALCIUM
卷 49, 期 4, 页码 226-232出版社
CHURCHILL LIVINGSTONE
DOI: 10.1016/j.ceca.2011.02.005
关键词
Platelet activation; Intracellular calcium; Proteasome; Calpain
类别
资金
- Council of Scientific and Industrial Research (CSIR)
- Department of Biotechnology (DBT)
- Department of Science and Technology (DST), Government of India
Ubiquitin-proteasome system has emerged a central player in regulation of diverse cellular processes. However, relevance of proteasome activity in platelets, which are terminally differentiated enucleate cells, is not clear. In this report we show that activation of platelets with physiological agonists was associated with 7-10 -fold rise in proteasomal activity. Elevation of cytosolic calcium with A23187 or thapsigargin resulted in significant increase in enzymatic activity, while treatment with intracellular calcium chelator or inhibitor of inositol trisphosphate receptor attenuated proteasomal enzymes in collagen-stimulated platelets. Specific inhibitors of protein kinase C as well as calpain, too, downregulated proteasome function. To conclude, proteasomal enzymatic activity in platelets is regulated by cytosolic calcium through Ca(2+)-dependent downstream effectors like calpain and protein kinase C. (C) 2011 Elsevier Ltd. All rights reserved.
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