期刊
CELL CALCIUM
卷 47, 期 1, 页码 65-76出版社
CHURCHILL LIVINGSTONE
DOI: 10.1016/j.ceca.2009.11.006
关键词
Mechanical stimulation; Purinergic receptor; Ca2+ wave; IP3 receptor; Store operated Ca2+ entry; Maxi-anion channel; Mitochondria
类别
资金
- NIH [RO1 DE14756, GM57249]
- NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH [R01DE014756] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM057249] Funding Source: NIH RePORTER
Coordination of Ca2+ signaling among cells contributes to synchronization of salivary gland cell function. However, mechanisms that underlie this signaling remain elusive. Here, intercellular Ca2+ waves (ICW) in submandibular gland cells were investigated using Fura-2 fluorescence imaging. Mechanical stimulation of single cells induced ICW propagation from the stimulated cells through similar to 7 layers of cells or similar to 120 mu m. Our findings indicate that an extracellular ATP-dependent pathway is involved because the purinergic receptor antagonist suramin and the ATP hydrolyzing enzyme apyrase blocked ICW propagation. However, the gap junction uncoupler oleamide had no effect. ATP is released from mechanically stimulated cells possibly through opening of mechanosensitive maxi-anion channels, and does not appear to be directly linked to cytosolic Ca2+. The ICW is propagated by diffusing ATP, which activates purinergic receptors in neighboring cells. This purinergic signaling induces a Ca2+ transient that is dependent on Ca2+ release via IP3 receptors in the ER and store operated Ca2+ entry (SOCE). Finally, inhibition of mitochondrial Ca2+ uptake modified ICW indicating an important role of these organelles in this phenomenon. These studies increase our understanding of purinergic receptor signaling in salivary gland cells, and its role as a coordination mechanism of Ca2+ signals induced by mechanical stimulation. (C) 2009 Elsevier Ltd. All rights reserved.
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