期刊
CELL CALCIUM
卷 48, 期 4, 页码 225-231出版社
CHURCHILL LIVINGSTONE
DOI: 10.1016/j.ceca.2010.09.004
关键词
Astroglia; Ca2+ signalling; NMDA receptors; P2X receptors; Synaptic transmission; Neuronal-glial communication
类别
资金
- BBSRC [BB/F021445]
- Grant Agency of the Czech Republic (GACR) [305/08/1381, 305/08/1384]
- BBSRC [BB/F021445/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/F021445/1] Funding Source: researchfish
Local, global and propagating calcium (Ca2+) signals provide the substrate for glial excitability. Here we analyse Ca2+ permeability of NMDA and P2X(1/5) receptors expressed in cortical astrocytes and provide evidence that activation of these receptors trigger astroglial Ca2+ signals when stimulated by either endogenous agonists or by synaptic release of neurotransmitters. The Ca2+ permeability of the ionotropic receptors was determined by reversal potential shift analysis; the permeability ratio P-Ca/P-K was 3.1 for NMDA receptors and 2.2 for P2X(1/5) receptors. Selective stimulation of ionotropic receptors (with NMDA and alpha,beta-methyleneATP) in freshly isolated cortical astrocytes induced ion currents associated with transient increases in cytosolic Ca2+ concentration ([Ca2+](i)). Stimulation of neuronal afferents in cortical slices triggered glial synaptic currents and [Ca2+](i) responses, which were partially blocked by selective antagonists of NMDA (D-AP5 and UBP141) and P2X(1/5) (NF449) receptors. We conclude that ionotropic receptors contribute to astroglial Ca2+ signalling and may provide a specific mechanism for fast neuronal-glial signalling at the synaptic level. (C) 2010 Elsevier Ltd. All rights reserved.
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