期刊
CELL CALCIUM
卷 45, 期 3, 页码 300-309出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ceca.2008.11.009
关键词
Drosophila photoreceptors; TRPL channel; TRPM7; Carvacrol; TRP inhibitors
类别
资金
- National Institute of Health [EY03529]
- Israel Science Foundation (ISF)
- US-Israel Binational Science Foundation (BSF)
- Moscona Foundation
- Minerva Foundation
Transient receptor potential (TRP) channels are essential components of biological sensors that detect changes in the environment in response to a myriad of stimuli. A major difficulty in the study of TRP channels is the lack of pharmacological agents that modulate most members of the TRIP superfamily. Notable exceptions are the thermoTRPs, which respond to either cold or hot temperatures and are modulated by a relatively large number of chemical agents. In the present study we demonstrate by patch clamp whole cell recordings from Schneider 2 and Drosophila photoreceptor cells that carvacrol, a known activator of the thermoTRPs, TRPV3 and TRPA1 is an inhibitor of the Drosophila TRPL channels, which belongs to the TRPC subfamily We also show that additional activators of TRPV3, thymol, eugenol, cinnamaldehyde and menthol are all inhibitors of the TRPL channel. Furthermore, carvacrol also inhibits the mammalian TRPM7 heterologously expressed in HEK cells and ectopically expressed in a primary culture of CA3-CA1 hippocampal brain neurons. This study, thus, identifies a novel inhibitor of TRPC and TRPM channels. Our finding that the activity of the non-thermoTRPs, TRPL and TRPM7 channels is modulated by the same compound as thermoTRPs, suggests that common mechanisms of channel modulation characterize TRP channels. (c) 2008 Elsevier Ltd. All rights reserved.
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