期刊
CELL CALCIUM
卷 44, 期 5, 页码 479-491出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ceca.2008.03.001
关键词
TRPC1; Orai; STIM1; Store-operated calcium influx; Receptor-operated calcium influx; Lipid rafts; Cholera toxin B subunit
类别
资金
- Consejo Nacional de Ciencia y Tecnotogia (CONACYT)
- Direccion General de Asuntos det Personal Academico (DGAPA)
White the role of members from the TRPC family of channels as receptor-operated channels (ROC) is well established and supported by numerous studies, the role of this family of channels as store-operated channels (SOC) has been the focus of a heated controversy over the last few years. In the present study, we have explored the modulation of STIM1 on human TRPC1 channel. We show that the association of STIM1 to TRPC1 favors the insertion of TRPC1 into lipid rafts, where TRPC1 functions as a SOC. In the absence of STIM1, TRPC1 associates to other members from the TRPC family of channels to form ROCs. A novel TIRFM-FRET method illustrates the relevance of the dynamic association between STIM1 and TRPC1 for the activation of SOC and the lipid raft localization of the STIM1-TRPC1 complex. This study provides new evidence about the dual activity of TRPC1 (forming ROC or SOC) and the partners needed to determine TRPC1 functional fate. It highlights also the role of plasma membrane microdomains and ER-PM junctions in modulating TRPC1 channel function and its association to STIM1. 0 2008 Elsevier Ltd. All rights reserved.
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