期刊
CELL CALCIUM
卷 43, 期 6, 页码 591-601出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ceca.2007.10.004
关键词
astrocytes; endoplasmic reticulum; electron microscopy; Ca2+ signaling; Zn2+; capacitative calcium entry
类别
Ca2+ signaling is the astrocyte form of excitability and the endoplasmic reticulum (ER) plays an important role as an intracellular Ca2+ store. Since the subcellular distribution of the ER influences Ca2+ signaling, we compared the arrangement of ER in astrocytes of hippocampus tissue and astrocytes in cell culture by electron microscopy. While the ER was usually located in close apposition to the plasma membrane in astrocytes in situ, the ER in cultured astrocytes was close to the nuclear membrane. Activation of metabotropic receptors linked to release of Ca2+ from ER stores triggered distinct responses in cultured and it? situ astrocytes. In culture, Ca2+ sionals were commonly first recorded close to the nucleus and with a delay at peripheral regions of the cells. Store-operated Ca2+ entry (SOC) as a route to refill the Ca2+ stores could be easily identified in cultured astrocytes as the Zn2+-sensitive component of the Ca2+ signal. In contrast, such a Zn2+-sensitive component was not recorded in astrocytes from hippocampal slices despite of evidence for SOC. Our data indicate that both, astrocytes in situ and in vitro express SOC necessary to refill stores, but that a SOC-related signal is not recorded in the cytoplasm of astrocytes in situ since the stores are close to the plasma membrane and the refill does not affect cytoplasmic Ca2+ levels. (C) 2007 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据