期刊
CELL BIOLOGY INTERNATIONAL
卷 37, 期 12, 页码 1267-1275出版社
WILEY
DOI: 10.1002/cbin.10141
关键词
dental pulp stem cells (DPSCs); nuclear factor kappaB (NF-B); osteogenic differentiation; tumour necrosis factor (TNF-alpha)
类别
资金
- Natural Science Foundation of China [81172841, 81202368]
- Natural Science Foundation of Jiangsu Colleges and Universities [09KJB320010, 10KJB320012]
- 'Top Six Types of Talents' Financial Assistance of Jiangsu Province
- Jiangsu province's Outstanding Medical Academic Leader Program [LJ201135, LJ201136]
- Science Foundation of Nantong city [H2011054]
- A project from Bureau of Jiangsu Province [Z2010005]
- Preventive Medicine Projects from Bureau of Jiangsu Province [Y2012083]
Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cells (MSCs) characterised by self-renewal and multi-lineage differentiation, including chondrocytes, adipocytes, neural cells and osteoblasts, which make it an attractive choice for tissue engineering purposes. Tumour necrosis factor (TNF-) had the positive effect on the mineralisation of bone marrow MSCs and stromal cells derived from human adipose tissue. However, the effect of TNF- on DPSCs is unclear. We found that TNF- activated the NF-B pathway during the osteogenic differentiation of DPSCs. TNF- also increased mineralisation and the expression of bone morphogenetic protein 2 (BMP2), alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2) and collagen type I (COL I) during this process. PDTC, an NF-B inhibitor, blocked the osteogenic differentiation induced by TNF-. No effect of TNF- on proliferation of DPSCs or cell cycle was detected. In summary, TNF- promotes mineralisation and mineralisation-related gene expression through the NF-B signalling pathway in DPSCs, which may provide a foundation for autologous transplantation of DPSCs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据