期刊
CELL BIOLOGY INTERNATIONAL
卷 38, 期 1, 页码 72-84出版社
WILEY-BLACKWELL
DOI: 10.1002/cbin.10181
关键词
cartilage tissue engineering; chitosan injectable hydrogel; chondrogenesis; in vitro release profile; mesenchymal stem cells
类别
资金
- Iranian Council of Stem Cell Technology [3001]
- Ferdowsi University of Mashhad [19723/3]
- Iranian Academic Center for Education, Culture and Research (ACECR)
- German Bundesministerium fur Bildung und Forschung [BCRT 0315848A]
Chitosan-beta glycerophosphate-hydroxyethyl cellulose (CH-GP-HEC) is a biocompatible and biodegradable scaffold exhibiting a sol-gel transition at 37 degrees C. Chondrogenic factors or mesenchymal stem cells (MSCs) can be included in the CH-GP-HEC, and injected into the site of injury to fill the cartilage tissue defects with minimal invasion and pain. The possible impact of the injectable CH-GP-HEC on the viability of the encapsulated MSCs was assessed by propidium iodide-fluorescein diacetate staining. Proliferation of the human and rat MSCs was also determined by MTS assay on days 0, 7, 14 and 28 after encapsulation. To investigate the potential application of CH-GP-HEC as a drug delivery device, the in vitro release profile of insulin was quantified by QuantiPro-BCA protein assay. Chondrogenic differentiation capacity of the encapsulated human MSCs (hMSCs) was also determined after induction of differentiation with transforming growth factor 3. MSCs have very good survival and proliferative rates within CH-GP-HEC hydrogel during the 28-day investigation. A sustained release of insulin occurred over 8 days. The CH-GP-HEC hydrogel also provided suitable conditions for chondrogenic differentiation of the encapsulated hMSCs. In conclusion, the high potential of CH-GP-HEC as an injectable hydrogel for cartilage tissue engineering is emphasised.
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