期刊
CELL BIOLOGY INTERNATIONAL
卷 35, 期 12, 页码 1261-1266出版社
WILEY
DOI: 10.1042/CBI20110026
关键词
alveolar epithelial cells; bone marrow mesenchymal stem cell (BMSC); differentiation; type II alveolar cells; surfactant protein C (SPC)
类别
资金
- National Natural Science Funds [30571843]
In this study, we demonstrate that BMSCs (bone marrow mesenchymal stem cells) can be successfully differentiated into type II alveolar epithelial cells in vitro under mimic pulmonary microenvironment. BMSCs were co-cultured with MAC-5 cells in modified SAGM (small airway growth medium). The BMSC-derived type II alveolar epithelial cells morphologically resemble human lung epithelial cells. They began to appear after 10 days in co-culture and became morphologically dominant after day 15. Correspondingly, SPC (surfactant protein C), a specific functional marker of human type II alveolar epithelial cells, was detected in differentiated cells by RT-PCR (reverse transcription-FOR) analysis after day 15. Immunostaining analysis revealed the present of scattered SPC-positive cells with a differentiation efficiency of 2.43-4.21%. Our study further showed that the SPC gene expression level in differentiated cells was related to the ratio of BMSCs to MRC-5 cells and the components of modified SAGM.
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