期刊
CELL BIOLOGY INTERNATIONAL
卷 36, 期 2, 页码 163-167出版社
PORTLAND PRESS LTD
DOI: 10.1042/CBI20100723
关键词
aloe emodin; apoptosis; calpain-2; hepatocellular carcinoma; Huh-7 cells; ubiquitin-protein ligase E3A
类别
资金
- Han Cell Inc. [2009-1-1]
- Agricultural R&D Promotion Center, Korea Rural Economic Institute, Republic of Korea [109085-3]
Natural flavonoids are associated with anti-proliferation of cancer growth. However, the antioxidant and anti-proliferation effects of AE (aloe-emodin) have not been well studied. We have investigated how AE affects the proliferation of hepatic hepatocellular carcinoma cells and exerts an anti-cancer effect. The cytotoxic effect of AE was demonstrated using an MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide] assay and Huh-7 cells were inhibited by AE treatment in both dose- and time-dependent manners. The IC50 level of AE was similar to 75 mu M. AE also has anti-proliferative effects via induction of DNA damage and apoptosis. 2-DE (two-dimensional electrophoresis) revealed that several proteins were related to the anti-cancer effects of AE. CAPN2 (calpain-2) and UBE3A (ubiquitin-protein ligase E3A), which are associated with the apoptosis signalling pathway, were verified by Western blotting. AE exhibited potent anti-proliferative effects on Huh-7 cells via down-regulation of CAPN2 and UBE3A. The findings support the possibility of AE being a chemopreventative agent.
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